The Museum displays an average of forty exhibits on three floors. Each floor is themed: permanent exhibits occupy "Our World" on the lower floor and "Our Town" on the main floor, while art exhibitions, science exhibits and traveling and/or temporary exhibits occupy the second floor. Permanent interactive exhibits include:
* Impressions Series: African Hut, Artifacts; Australian Lingo Game, Artwork; Asian Tea House
* Theatre: Stage, Puppets, Costumes, Scripts, Musical Instruments, Theatre Seating
* "O" is for Orange - from Seed to Our World: Florida Orange Grove and Juice Factory
* Water Matters: Working Model of Earth's Continuous Water Cycle
* News Station: Weather Instruments, News Desk, Scripts, Cameras, Video Screens
* Marvelous Me!: Health & Wellness Clinic with Healthcare Equipment, Instruments
* Explore Store: Grocery Store with Working Registers, Stocked Shelves, Grocery Carts, Career Kiosk
* Mega Mike: Wentzscope Microscope, Magnifying Bar, Magnifying Glasses
* Transportation Vehicles: Fire Engine, Sheriff's Car, Airplane, Boat, Hot Air Balloon, Space Shuttle
* Florida Cottage: Facade & Porch, Rocker, Home Implements
* Raceways: The Physics of Movement & Design with Ball Runs, Rubber Band Design
* Go Green!: Recycling House
* Tot Spot: Educational Toys, Books, Tree House
Noteworthy pieces curated by the Museum include:
*"Celebrating Who I Am", a VSA collection of artwork by youth artists with disabilities, one from each state, acquired from the Kennedy Center
* "Animation Station", a collection of forty-one original Disney animation cels, acquired from Walt Disney World
* "Swanna Play?", a three-dimensional five foot tall fiberglass swan commissioned by the Museum as part of the highly successful community public art project and fundraiser "Swansation"
* "D.O.T., the Dragon of Toys", a three-dimensional thirty foot long sculpture of a whimsical dragon made of recycled toys which hovers above the main floor to greet visitors as they enter the Museum
* "Impressions ", authentic cultural artifacts from Japan and Africa in our "Impressions" Gallery
*"Families through the Decades" and "People & Animals of the World", wall murals painted by a professional artist